Download, install, and get started using the DNAnexus Platform SDK, the DNAnexus upload and download agents, and dxCompiler.

DNAnexus Platform SDK

The DNAnexus Platform SDK - also called dx-toolkit - includes the dx command-line client; tools for building and debugging apps; utilities for working with DNA data on the DNAnexus Platform; and Python, Java, C++ and R bindings for working on the DNAnexus Platform.
The SDK is released under the Apache v2.0 license.
If you plan to rely on the user interface to interact with the Platform, using the command-line interface only to upload files, you likely don't need to download the full SDK. The Upload Agent may be enough to meet your needs.

Installing the Python SDK and Command Line Tools

Before You Begin

Before installing the Python SDK and command line tools, ensure that you are running Python 3.


The SDK's Python components - dxpy bindings and command line tools, including dx- are distributed through PyPI. Use pip3 to download and install the dxpy package:
pip3 install dxpy
If you see a warning similar to the one below, please update your PATH environment variable to include the path where pip3 installed the new commands.
WARNING: The scripts dx, dx-app-wizard, dx-build-app and dx-build-applet are installed in
'/Users/user-amy/Library/Python/3.9/bin' which is not on PATH.
Consider adding this directory to PATH

Enabling Tab Completion

If you're using Z shell (zsh), which is included in recent versions of OS X, enable tab completion by entering the following command, or adding it to your .zshrc:
autoload -Uz compinit && compinit
autoload bashcompinit && bashcompinit
eval "$(register-python-argcomplete dx|sed 's/-o default//')"
If you're not using Z shell (zsh), enable tab completion by entering the following command, or add it to your .bashrc:
eval "$(register-python-argcomplete dx|sed 's/-o default//')"

Upgrading dxpy

To upgrade the dxpypackage after installing it using pip3, enter the command:
pip3 install --upgrade dxpy.
Only use dx upgrade with tarball installations as described below.


If you're using the OS X Xcode command line tools and running Python 3, the psutil dependency may fail to compile. To prevent this from happening, set export ARCHFLAGS="-arch x86_64" before installing dxpy.
To avoid character set problems when running the dx command, set the PYTHONIOENCODING environment variable to UTF-8 as follows:

Installing the Java, R, and C++ SDK

The Java, R, and C++ SDK is available for the following platforms:
Ubuntu Linux 20.04
CentOS, Red Hat Enterprise Linux, Scientific Linux 5/6/7
Mac OS X

Installation on Linux or OS X

Start by unpacking the SDK:
$ tar -xzf dx-toolkit*.tar.gz
Next, if you are using zsh on OS X, update your~/.zshrc file as follows:
echo "autoload -Uz compinit && compinit" >> ~/.zshrc && source ~/.zshrc
Then initialize your environment:
$ cd dx-toolkit && source environment

Installation on Windows

Windows 10 and later versions are supported.
Start the installation by running the command dx-toolkit*.exe.
Systems with Windows Defender enabled may present a warning before launching the dx-toolkit installer. If you see this warning window, click More Info, then choose Run Anyway.
After the installation is complete, find and click on the DNAnexus CLI shortcut on the Windows Start Menu.
The Windows installer source code is available from the dx-toolkit Github repository.
If your organization does not allow installation on Windows systems without the installer package's signature, contact DNAnexus Support to learn about alternative installation methods.
The Python package "python-certifi-win32" is needed (use pip install python-certifi-win32 command to install it) if you encounter the following errors during certain operations (e.g. dx head file-xxx):
ssl.SSLCertVerificationError: [SSL: CERTIFICATE_VERIFY_FAILED] certificate verify failed: unable to get local issuer certificate (_ssl.c:1108)

Upgrading a Tarball Installation

If you installed the SDK via a tarball, run the dx upgrade command, without arguments, to upgrade to the latest recommended version:
$ dx upgrade
Upgrading to v0.325.1
Downloading dx-toolkit-v0.325.1-osx.tar.gz (using curl -O -L)...
% Total % Received % Xferd Average Speed Time Time Time Current
Dload Upload Total Spent Left Speed
100 9874k 100 9874k 0 0 3279k 0 0:00:03 0:00:03 --:--:-- 3279k
Unpacking dx-toolkit-v0.325.1-osx.tar.gz...
Backing up version v0.227.1...
Moving version v0.306.0 into place... Updated to version v0.306.0. Previous version saved in /Users/user-amy/dnanexus/dx-toolkit/build/v0.227.1.
Please close this terminal, open a new terminal, and source the environment file in the dx-toolkit folder

Reverting a Tarball Installation to a Previous Version

You can also run dx upgrade if you want to revert to a previous version of the SDK, by adding the version number as an argument:
$ dx upgrade v0.225.0

Building from Source

Download either of the following source packages:
Source package .tar.gz
Source package .zip
See the file in the archive, or in the dx-toolkit Github repository, for information on build-time dependencies, as well as additional installation instructions.

Using the SDK

Using a Proxy

To direct dx-toolkit to a proxy, set the HTTP_PROXY or HTTPS_PROXY environment variable to an appropriate value:
export HTTP_PROXY=hostname:port

TLS Settings

By default, dx-toolkit uses certificates installed on your system to create secure connections. If your system requires an additional TLS certificate, and dx-toolkit doesn't appear to be using a certificate installed on your system, you have two options. The first, and most secure, is to set the DX_CA_CERT environment variable to the path of the PEM-encoded TLS certificate file required by your parent organization. The second option is to connect insecurely, avoiding certificate verification altogether, by setting DX_CA_CERT=NOVERIFY.
When using Windows, environments can be customized in a wide variety of ways. As a result, issues can arise even when using the TLS settings detailed in the previous paragraph. Contact DNAnexus Support if you are using Windows and have trouble connecting through a proxy.

Learn More

  • The Command Line Quickstart will get you started on accessing the platform via the command-line interface.
  • The Developer section contains comprehensive information on how to use the SDK to interact with, and write your own applications for the Platform.

Upload Agent

The DNAnexus Upload Agent is a command-line tool for uploading files to the DNAnexus Platform. Using the Upload Agent is a fast and convenient alternative to uploading files via the Platform UI.
Using the Upload Agent requires an authentication token. Please create an authentication token before proceeding.


  • Fast Parallel Uploads - Data is uploaded using multiple parallel connections for faster upload speeds.
  • Automatic Compression - Text files (such as FASTA, FASTQ, qseq, and other text files) can be automatically compressed before upload, for greater efficiency.
  • Automated Uploads - No user interaction is required; the client can be used to automate uploads of entire sequencing runs.
  • Resumable Uploads - Previously interrupted uploads can be automatically resumed.
  • Progress Reporting - Users can monitor the progress of individual file uploads, as well as average/instantaneous transfer rates.

Installing the Upload Agent

The Upload Agent is available pre-built for 64-bit Linux, OS X, and Windows.

Installation on 64-bit Linux

Install the Upload Agent on 64-bit Linux as follows:
wget \ -O - |\
tar -xzf -
cd dnanexus-upload-agent-*-linux

Installation on OS X

Install the Upload Agent on OS X as follows:
curl \ \
cd dnanexus-upload-agent-*-osx

Installation on Windows

To install the Upload Agent on Windows:
  1. 2.
    Unzip the file using your favorite utility
  2. 3.
    Run ua.exe

Using the Upload Agent

Download Agent

The DNAnexus Download Agent is a command-line tool for downloading large numbers of files from the DNAnexus Platform to a local environment. It is designed to ensure the success of downloads that can take a long time to complete.

Features and Usage

For a detailed description of Download Agent features and usage, please review the README file in the Download Agent Github repository.


A list of Download Agent releases is available in Releases section of the Download Agent Github repository. The Download Agent Docker repository provides access to experimental builds corresponding to commits to the master branch.


dxCompiler is used to generate a DNAnexus workflow from a workflow written in Workflow Description Language (WDL). dxCompiler is a JAR file that can be run with Java 8 or later, and can be downloaded from the releases section of the dxCompiler Github repository. dxCompiler is also released as a public Docker container that includes the Java dependency. The dxCompiler README documents setup instructions and sample workflows.
Before using dxCompiler, ensure that you do the following:
  • Install the dxcommand-line tool
  • Initialize the dxcommand-line tool via the commanddx login
  • Either
    • Install the Java SE Development Kit 8 (or above). The Java SE Development Kit can be use to invoke dxCompiler via the command java -jar dxCompiler.jar compile workflow_file.wdl
    • Install the Docker command-line tool. It can then be used to invoke dxCompiler inside the Docker container, using the run-dxcompiler-docker script.